Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2974761..2975409 Replicon chromosome
Accession NZ_CP010339
Organism Mycobacterium tuberculosis strain 22103

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag RN14_RS13940 Protein ID WP_003899414.1
Coordinates 2974761..2975084 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag RN14_RS13945 Protein ID WP_003899415.1
Coordinates 2975164..2975409 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN14_RS13910 (RN14_2813) 2970084..2971082 + 999 WP_034163937.1 tyrosine-type recombinase/integrase -
RN14_RS13915 (RN14_2814) 2971096..2971560 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
RN14_RS13920 2971548..2971799 + 252 WP_003908028.1 hypothetical protein -
RN14_RS13925 (RN14_2816) 2971970..2973409 - 1440 WP_003899411.1 phage major capsid protein -
RN14_RS13930 (RN14_2817) 2973417..2973950 - 534 WP_003899412.1 HK97 family phage prohead protease -
RN14_RS21420 (RN14_2818) 2974103..2974594 - 492 WP_003900541.1 phage terminase small subunit P27 family -
RN14_RS13940 (RN14_2819) 2974761..2975084 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
RN14_RS13945 (RN14_2820) 2975164..2975409 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
RN14_RS13950 (RN14_2821) 2975406..2976833 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
RN14_RS13955 (RN14_2822) 2976835..2977227 - 393 WP_003899417.1 DUF2742 domain-containing protein -
RN14_RS13960 (RN14_2823) 2977224..2977484 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
RN14_RS13965 (RN14_2824) 2977501..2977863 - 363 WP_003900543.1 hypothetical protein -
RN14_RS13970 (RN14_2825) 2977866..2978993 - 1128 WP_003899420.1 site-specific integrase -
RN14_RS13975 2979138..2979365 - 228 WP_003899421.1 hypothetical protein -
RN14_RS21425 2979362..2979751 - 390 WP_003899422.1 hypothetical protein -
RN14_RS13980 (RN14_2827) 2979657..2979929 + 273 WP_003900544.1 hypothetical protein -
RN14_RS13985 (RN14_2828) 2980028..2980261 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2970084..2978993 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T52160 WP_003899414.1 NZ_CP010339:c2975084-2974761 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T52160 NZ_CP010339:c2975084-2974761 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT52160 WP_003899415.1 NZ_CP010339:c2975409-2975164 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT52160 NZ_CP010339:c2975409-2975164 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTCTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References