Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2985598..2986246 Replicon chromosome
Accession NZ_CP010330
Organism Mycobacterium tuberculosis strain F28

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag RN05_RS14130 Protein ID WP_003899414.1
Coordinates 2985598..2985921 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag RN05_RS14135 Protein ID WP_003899415.1
Coordinates 2986001..2986246 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RN05_RS14110 2981172..2982433 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
RN05_RS22560 2982460..2982645 - 186 WP_009935348.1 hypothetical protein -
RN05_RS14115 2982807..2984246 - 1440 WP_009935349.1 phage major capsid protein -
RN05_RS14120 2984254..2984787 - 534 WP_003899412.1 HK97 family phage prohead protease -
RN05_RS21670 2984940..2985431 - 492 WP_003900541.1 phage terminase small subunit P27 family -
RN05_RS14130 2985598..2985921 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
RN05_RS14135 2986001..2986246 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
RN05_RS14140 2986243..2987670 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
RN05_RS14145 2987672..2988064 - 393 WP_003899417.1 DUF2742 domain-containing protein -
RN05_RS14150 2988061..2988321 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
RN05_RS14155 2988338..2988700 - 363 WP_003900543.1 hypothetical protein -
RN05_RS14160 2988703..2989830 - 1128 WP_003899420.1 site-specific integrase -
RN05_RS14165 2989975..2990202 - 228 WP_003899421.1 hypothetical protein -
RN05_RS21675 2990199..2990588 - 390 WP_003899422.1 hypothetical protein -
RN05_RS14170 2990494..2990766 + 273 WP_003900544.1 hypothetical protein -
RN05_RS14175 2990865..2991098 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2979563..2989830 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T52099 WP_003899414.1 NZ_CP010330:c2985921-2985598 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T52099 NZ_CP010330:c2985921-2985598 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT52099 WP_003899415.1 NZ_CP010330:c2986246-2986001 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT52099 NZ_CP010330:c2986246-2986001 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References