Detailed information of TA system TA052041

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24844..25108
• Replicon: plasmid pAPEC-O78-2
• Accession: NZ_CP010317
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: AC789_RS25715
• Protein ID: WP_001303307.1
• Coordinates: 24844..24996 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	25046..25108 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS25685	19847..22015	+	2169	WP_000698368.1	DotA/TraY family protein	-
AC789_RS25690	22086..22748	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
AC789_RS25695	22820..23029	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AC789_RS30110	23421..23597	+	177	WP_001054900.1	hypothetical protein	-
-	24083..24134	+	52	NuclAT_1	-	-
-	24083..24134	+	52	NuclAT_1	-	-
-	24083..24134	+	52	NuclAT_1	-	-
-	24083..24134	+	52	NuclAT_1	-	-
AC789_RS25710	24521..24772	+	252	WP_001291965.1	hypothetical protein	-
AC789_RS25715	24844..24996	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	25046..25108	+	63	NuclAT_0	-	Antitoxin
-	25046..25108	+	63	NuclAT_0	-	Antitoxin
-	25046..25108	+	63	NuclAT_0	-	Antitoxin
-	25046..25108	+	63	NuclAT_0	-	Antitoxin
AC789_RS25720	25288..26496	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
AC789_RS25725	26515..27585	+	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
AC789_RS25730	27578..29869	+	2292	WP_001289269.1	TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..106397	106397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T52041 WP_001303307.1 NZ_CP010317:c24996-24844 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T52041 NZ_CP010317:c24996-24844 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT52041 NZ_CP010317:25046-25108 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin