Detailed information of TA system TA052031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3111282..3112009
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: AC789_RS15330
• Protein ID: WP_000547564.1
• Coordinates: 3111698..3112009 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AC789_RS15325
• Protein ID: WP_000126294.1
• Coordinates: 3111282..3111701 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS15305	3106332..3106859	-	528	WP_001078777.1	electron transport protein HydN	-
AC789_RS15310	3107008..3108018	-	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
AC789_RS15315	3108278..3109735	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AC789_RS15320	3109744..3111168	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
AC789_RS15325	3111282..3111701	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AC789_RS15330	3111698..3112009	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
AC789_RS15335	3112183..3112944	-	762	WP_001026446.1	hypothetical protein	-
AC789_RS15340	3112969..3113439	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AC789_RS15345	3113432..3113842	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
AC789_RS15350	3113839..3114606	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AC789_RS15355	3114606..3115148	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
AC789_RS15360	3115158..3116867	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T52031 WP_000547564.1 NZ_CP010315:c3112009-3111698 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T52031 NZ_CP010315:c3112009-3111698 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT52031 WP_000126294.1 NZ_CP010315:c3111701-3111282 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT52031 NZ_CP010315:c3111701-3111282 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5