Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2352578..2353080 Replicon chromosome
Accession NZ_CP010315
Organism Escherichia coli strain 789

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag AC789_RS11715 Protein ID WP_000767820.1
Coordinates 2352578..2352832 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AC789_RS11720 Protein ID WP_001259255.1
Coordinates 2352829..2353080 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AC789_RS11690 2347593..2347820 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AC789_RS11695 2347834..2348892 - 1059 WP_000492324.1 transport protein YeeE -
AC789_RS11700 2349071..2350429 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AC789_RS29450 2350419..2350481 - 63 WP_010723108.1 membrane protein YoeI -
AC789_RS11705 2350696..2351625 - 930 WP_000803351.1 LysR family transcriptional regulator -
AC789_RS11710 2351671..2352495 - 825 WP_000754756.1 SDR family oxidoreductase -
AC789_RS11715 2352578..2352832 - 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AC789_RS11720 2352829..2353080 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AC789_RS27335 2353364..2353414 + 51 WP_001364200.1 his operon leader peptide -
AC789_RS11725 2353560..2354459 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AC789_RS11730 2354465..2355769 + 1305 WP_039025710.1 histidinol dehydrogenase -
AC789_RS11735 2355766..2356836 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
AC789_RS11740 2356836..2357903 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T52029 WP_000767820.1 NZ_CP010315:c2352832-2352578 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T52029 NZ_CP010315:c2352832-2352578 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT52029 WP_001259255.1 NZ_CP010315:c2353080-2352829 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT52029 NZ_CP010315:c2353080-2352829 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References