Detailed information of TA system TA052015

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1493560..1493931
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: AC789_RS07335
• Protein ID: WP_001317028.1
• Coordinates: 1493737..1493931 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1493560..1493738 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS07310	1489515..1490888	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
AC789_RS07315	1491017..1491952	-	936	WP_001157405.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AC789_RS26935	1492004..1493239	-	1236	WP_071925352.1	site-specific integrase	-
AC789_RS07325	1493241..1493456	-	216	WP_000079604.1	excisionase XisR	-
AC789_RS07330	1493535..1493744	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1493560..1493738	+	179	NuclAT_0	-	Antitoxin
-	1493560..1493738	+	179	NuclAT_0	-	Antitoxin
-	1493560..1493738	+	179	NuclAT_0	-	Antitoxin
-	1493560..1493738	+	179	NuclAT_0	-	Antitoxin
AC789_RS07335	1493737..1493931	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AC789_RS07340	1493988..1494797	-	810	WP_000166319.1	recombination protein RecT	-
AC789_RS07345	1494790..1497390	-	2601	WP_044502199.1	exodeoxyribonuclease VIII	-
AC789_RS07350	1497492..1497767	-	276	WP_000632297.1	protein RacC	-
AC789_RS07355	1497842..1498012	-	171	WP_077790356.1	hypothetical protein	-
AC789_RS07360	1498012..1498233	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1492004..1541726	49722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T52015 WP_001317028.1 NZ_CP010315:c1493931-1493737 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T52015 NZ_CP010315:c1493931-1493737 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT52015 NZ_CP010315:1493560-1493738 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin