Detailed information of TA system TA051980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1698900..1699481
• Replicon: chromosome
• Accession: NZ_CP010311
• Organism: Geoalkalibacter subterraneus strain Red1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B5FE87
• Locus tag: GSUB_RS07825
• Protein ID: WP_040200104.1
• Coordinates: 1699203..1699481 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GSUB_RS07820
• Protein ID: WP_040200103.1
• Coordinates: 1698900..1699187 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSUB_RS07780	1694061..1694819	+	759	WP_040200094.1	5'/3'-nucleotidase SurE	-
GSUB_RS07785	1694914..1695567	+	654	WP_040200096.1	protein-L-isoaspartate(D-aspartate) O-methyltransferase	-
GSUB_RS07790	1695573..1696166	+	594	WP_144401981.1	DedA family protein	-
GSUB_RS18565	1696239..1697006	+	768	WP_158414059.1	peptidoglycan DD-metalloendopeptidase family protein	-
GSUB_RS07805	1697064..1698098	+	1035	WP_040200101.1	sigma-70 family RNA polymerase sigma factor	-
GSUB_RS07810	1698154..1698672	+	519	WP_040200102.1	adenine phosphoribosyltransferase	-
GSUB_RS07820	1698900..1699187	-	288	WP_040200103.1	HigA family addiction module antidote protein	Antitoxin
GSUB_RS07825	1699203..1699481	-	279	WP_040200104.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GSUB_RS07830	1699726..1700352	+	627	WP_040202263.1	LysE family translocator	-
GSUB_RS07835	1700367..1701539	+	1173	WP_040202264.1	ATP-binding protein	-
GSUB_RS07840	1701557..1702363	+	807	WP_040200105.1	VWA domain-containing protein	-
GSUB_RS07845	1702459..1703433	+	975	WP_040200106.1	calcium/sodium antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10585.13 Da
• Isoelectric Point: 10.0345

>T51980 WP_040200104.1 NZ_CP010311:c1699481-1699203 [Geoalkalibacter subterraneus]
MIQTFRHKGLRQFYESGSKAGIVASQAKRLKLVLARLEAAREPRDMNLPGLRLHQMTGDLTGFWSVSVSGNWRVIFRFDG
PDVFDVDYLDYH

Nucleotide

• Length: 279 bp

>T51980 NZ_CP010311:c1699481-1699203 [Geoalkalibacter subterraneus]
ATGATTCAGACTTTCCGCCACAAGGGCCTCAGGCAGTTTTACGAATCAGGAAGCAAGGCTGGGATTGTCGCCAGCCAGGC
GAAGAGGCTTAAACTTGTCCTTGCCCGTCTCGAAGCCGCCCGCGAACCTCGCGACATGAACCTTCCTGGTTTGCGACTGC
ACCAGATGACGGGCGACCTTACCGGTTTCTGGTCGGTCAGCGTGAGCGGAAACTGGCGGGTGATCTTTCGCTTCGACGGA
CCGGATGTTTTCGACGTGGATTACCTGGACTATCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10441.21 Da
• Isoelectric Point: 9.0655

>AT51980 WP_040200103.1 NZ_CP010311:c1699187-1698900 [Geoalkalibacter subterraneus]
MMHNPPHPGEVIRELCLEPLGVTVTDAAKALGVSRKALSELLNGKTGVSPEMALRLSIAFDTTPESWLTQQMHYDLWQAK
KKAKGLKVEKMQTAA

Nucleotide

• Length: 288 bp

>AT51980 NZ_CP010311:c1699187-1698900 [Geoalkalibacter subterraneus]
ATGATGCACAACCCACCCCATCCAGGTGAAGTGATCCGTGAGCTTTGCCTTGAACCTCTCGGTGTGACTGTCACCGATGC
GGCCAAGGCACTCGGCGTGTCGAGAAAAGCTCTCTCTGAGCTGCTGAATGGCAAGACAGGGGTTAGTCCGGAAATGGCGC
TGCGTCTTTCTATCGCATTCGACACAACCCCGGAAAGCTGGCTGACTCAGCAGATGCACTATGACCTCTGGCAGGCGAAA
AAGAAGGCCAAGGGGCTCAAAGTAGAAAAAATGCAAACCGCAGCATAG

Structures

Toxin

• AlphaFold DB: A0A0B5FE87