Detailed information of TA system TA051948

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2063090..2063315
• Replicon: chromosome
• Accession: NZ_CP010304
• Organism: Escherichia coli O157:H7 str. SS52

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: SS52_RS10460
• Protein ID: WP_000813258.1
• Coordinates: 2063090..2063245 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2063257..2063315 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS52_RS10410	2058093..2058524	-	432	WP_001477269.1	tellurite resistance TerB family protein	-
SS52_RS10425	2058975..2059688	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
SS52_RS32740	2059824..2060021	-	198	WP_000917763.1	hypothetical protein	-
SS52_RS10435	2060246..2060800	-	555	WP_000640035.1	DUF1133 family protein	-
SS52_RS10440	2060863..2061168	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
SS52_RS10445	2061181..2062230	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
SS52_RS10450	2062232..2062504	-	273	WP_000191872.1	hypothetical protein	-
SS52_RS10455	2062626..2062970	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
SS52_RS10460	2063090..2063245	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2063257..2063315	+	59	-	-	Antitoxin
SS52_RS10465	2063536..2064093	-	558	WP_000104474.1	DUF551 domain-containing protein	-
SS52_RS10470	2064095..2064313	-	219	WP_000683609.1	DUF4014 family protein	-
SS52_RS10475	2064441..2064752	-	312	WP_001289673.1	hypothetical protein	-
SS52_RS10480	2064745..2064972	-	228	WP_000699809.1	hypothetical protein	-
SS52_RS10485	2064969..2065250	-	282	WP_000603384.1	DNA-binding protein	-
SS52_RS10490	2065283..2065999	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
SS52_RS32745	2066033..2066494	-	462	WP_000139447.1	replication protein	-
SS52_RS32750	2066487..2067530	-	1044	WP_001262402.1	hypothetical protein	-
SS52_RS10505	2067599..2068024	-	426	WP_000693878.1	Rha family transcriptional regulator	-
SS52_RS10510	2068008..2068250	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2014952..2136829	121877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T51948 WP_000813258.1 NZ_CP010304:c2063245-2063090 [Escherichia coli O157:H7 str. SS52]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T51948 NZ_CP010304:c2063245-2063090 [Escherichia coli O157:H7 str. SS52]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT51948 NZ_CP010304:2063257-2063315 [Escherichia coli O157:H7 str. SS52]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin