Detailed information of TA system TA051938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292451..293145
• Replicon: chromosome
• Accession: NZ_CP010304
• Organism: Escherichia coli O157:H7 str. SS52

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: SS52_RS01365
• Protein ID: WP_001263495.1
• Coordinates: 292747..293145 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: SS52_RS01360
• Protein ID: WP_000554757.1
• Coordinates: 292451..292744 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS52_RS32395	288162..288659	+	498	Protein_251	transposase	-
SS52_RS01345	288804..290516	-	1713	Protein_252	flagellar biosynthesis protein FlhA	-
SS52_RS01350	290488..291273	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
SS52_RS01355	291344..292399	+	1056	WP_001226186.1	DNA polymerase IV	-
SS52_RS01360	292451..292744	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
SS52_RS01365	292747..293145	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
SS52_RS01370	293155..293607	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
SS52_RS01375	293926..294129	+	204	Protein_258	RNA ligase RtcB family protein	-
SS52_RS01380	294128..294649	+	522	Protein_259	peptide chain release factor H	-
SS52_RS01385	294706..296163	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
SS52_RS01390	296424..296882	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	297478..297558	+	81	NuclAT_11	-	-
-	297478..297558	+	81	NuclAT_11	-	-
-	297478..297558	+	81	NuclAT_11	-	-
-	297478..297558	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	287213..331190	43977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T51938 WP_001263495.1 NZ_CP010304:292747-293145 [Escherichia coli O157:H7 str. SS52]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51938 NZ_CP010304:292747-293145 [Escherichia coli O157:H7 str. SS52]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51938 WP_000554757.1 NZ_CP010304:292451-292744 [Escherichia coli O157:H7 str. SS52]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51938 NZ_CP010304:292451-292744 [Escherichia coli O157:H7 str. SS52]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6