Detailed information of TA system TA051937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 286488..287027
• Replicon: chromosome
• Accession: NZ_CP010304
• Organism: Escherichia coli O157:H7 str. SS52

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: SS52_RS01325
• Protein ID: WP_001303998.1
• Coordinates: 286488..286748 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: SS52_RS01330
• Protein ID: WP_000729705.1
• Coordinates: 286767..287027 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SS52_RS01310	284171..284749	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
SS52_RS01315	284854..285621	+	768	WP_001301698.1	class II glutamine amidotransferase	-
SS52_RS01320	285592..286332	-	741	WP_001225679.1	murein L,D-transpeptidase	-
SS52_RS01325	286488..286748	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
SS52_RS01330	286767..287027	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
SS52_RS01335	287213..287986	+	774	WP_000543891.1	C40 family peptidase	-
SS52_RS32395	288162..288659	+	498	Protein_251	transposase	-
SS52_RS01345	288804..290516	-	1713	Protein_252	flagellar biosynthesis protein FlhA	-
SS52_RS01350	290488..291273	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T51937 WP_001303998.1 NZ_CP010304:c286748-286488 [Escherichia coli O157:H7 str. SS52]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T51937 NZ_CP010304:c286748-286488 [Escherichia coli O157:H7 str. SS52]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT51937 WP_000729705.1 NZ_CP010304:c287027-286767 [Escherichia coli O157:H7 str. SS52]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT51937 NZ_CP010304:c287027-286767 [Escherichia coli O157:H7 str. SS52]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA