Detailed information of TA system TA051762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1383863..1384465
• Replicon: chromosome
• Accession: NZ_CP010242
• Organism: Escherichia coli strain S56

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG73_RS07310
• Protein ID: WP_000897305.1
• Coordinates: 1383863..1384174 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG73_RS07315
• Protein ID: WP_000356397.1
• Coordinates: 1384175..1384465 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG73_RS07280	1378893..1379678	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG73_RS07285	1379777..1380376	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG73_RS07290	1380370..1381242	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG73_RS07295	1381239..1381676	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG73_RS07300	1381721..1382662	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
RG73_RS07305	1382726..1383634	-	909	WP_001299484.1	alpha/beta hydrolase	-
RG73_RS07310	1383863..1384174	+	312	WP_000897305.1	hypothetical protein	Toxin
RG73_RS07315	1384175..1384465	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG73_RS07325	1385070..1385288	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG73_RS07330	1385507..1385749	+	243	WP_001087409.1	hypothetical protein	-
RG73_RS07340	1386079..1387008	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG73_RS07345	1387005..1387640	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG73_RS07350	1387637..1388539	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51762 WP_000897305.1 NZ_CP010242:1383863-1384174 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51762 NZ_CP010242:1383863-1384174 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51762 WP_000356397.1 NZ_CP010242:1384175-1384465 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51762 NZ_CP010242:1384175-1384465 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7