Detailed information of TA system TA051754

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48947..49368
• Replicon: plasmid A
• Accession: NZ_CP010241
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: RG29_RS28945
• Protein ID: WP_001302184.1
• Coordinates: 48947..49105 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	49170..49368 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG29_RS28905	44336..45033	+	698	Protein_46	IS1-like element IS1A family transposase	-
RG29_RS28910	45201..45584	-	384	WP_001063020.1	relaxosome protein TraM	-
RG29_RS28920	45936..46526	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
RG29_RS28925	46822..47643	-	822	WP_001234417.1	DUF945 domain-containing protein	-
RG29_RS28930	47762..48049	-	288	WP_000107542.1	hypothetical protein	-
RG29_RS30525	48074..48280	-	207	WP_000547965.1	hypothetical protein	-
RG29_RS30530	48350..48709	+	360	Protein_52	hypothetical protein	-
RG29_RS28945	48947..49105	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49170..49368	-	199	NuclAT_0	-	Antitoxin
-	49170..49368	-	199	NuclAT_0	-	Antitoxin
-	49170..49368	-	199	NuclAT_0	-	Antitoxin
-	49170..49368	-	199	NuclAT_0	-	Antitoxin
RG29_RS28955	49380..50099	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
RG29_RS28960	50096..50530	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
RG29_RS28965	50585..52543	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
RG29_RS28970	52609..52842	-	234	WP_000005990.1	DUF905 family protein	-
RG29_RS28975	52899..53351	-	453	WP_000290802.1	single-stranded DNA-binding protein	-
RG29_RS30535	53669..54178	+	510	WP_032163513.1	hypothetical protein	-
RG29_RS28995	54148..54339	-	192	WP_001027495.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeC / faeD / faeE / faeF / faeH	1..62376	62376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T51754 WP_001302184.1 NZ_CP010241:c49105-48947 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51754 NZ_CP010241:c49105-48947 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT51754 NZ_CP010241:c49368-49170 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin