Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4216307..4216809 Replicon chromosome
Accession NZ_CP010240
Organism Escherichia coli strain C7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG29_RS22770 Protein ID WP_000767829.1
Coordinates 4216307..4216561 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG29_RS22775 Protein ID WP_001259255.1
Coordinates 4216558..4216809 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG29_RS22745 4211322..4211549 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG29_RS22750 4211563..4212621 - 1059 WP_000492340.1 transport protein YeeE -
RG29_RS22755 4212800..4214158 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG29_RS30180 4214148..4214210 - 63 WP_010723108.1 membrane protein YoeI -
RG29_RS22760 4214425..4215354 - 930 WP_000803360.1 LysR family transcriptional regulator -
RG29_RS22765 4215400..4216224 - 825 WP_000754737.1 SDR family oxidoreductase -
RG29_RS22770 4216307..4216561 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG29_RS22775 4216558..4216809 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG29_RS22780 4217092..4217142 + 51 WP_001364200.1 his operon leader peptide -
RG29_RS22790 4217288..4218187 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG29_RS22795 4218193..4219497 + 1305 WP_021547561.1 histidinol dehydrogenase -
RG29_RS22800 4219494..4220564 + 1071 WP_001468894.1 histidinol-phosphate transaminase -
RG29_RS22805 4220564..4221631 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51748 WP_000767829.1 NZ_CP010240:c4216561-4216307 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51748 NZ_CP010240:c4216561-4216307 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51748 WP_001259255.1 NZ_CP010240:c4216809-4216558 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51748 NZ_CP010240:c4216809-4216558 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References