Detailed information of TA system TA051721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1485910..1486512
• Replicon: chromosome
• Accession: NZ_CP010240
• Organism: Escherichia coli strain C7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG29_RS07735
• Protein ID: WP_000897305.1
• Coordinates: 1486201..1486512 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG29_RS07730
• Protein ID: WP_000356397.1
• Coordinates: 1485910..1486200 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG29_RS07695	1481476..1482111	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG29_RS07700	1482108..1483037	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG29_RS07705	1483436..1484416	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
RG29_RS07715	1484645..1484887	-	243	WP_001086388.1	hypothetical protein	-
RG29_RS07720	1485106..1485324	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG29_RS07730	1485910..1486200	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG29_RS07735	1486201..1486512	-	312	WP_000897305.1	hypothetical protein	Toxin
RG29_RS07740	1486741..1487649	+	909	WP_001371792.1	alpha/beta hydrolase	-
RG29_RS07745	1487713..1488654	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
RG29_RS07750	1488699..1489136	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG29_RS07755	1489133..1490005	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG29_RS07760	1489999..1490598	-	600	WP_001295269.1	glucose-1-phosphatase	-
RG29_RS07765	1490697..1491482	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1483436..1484416	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51721 WP_000897305.1 NZ_CP010240:c1486512-1486201 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51721 NZ_CP010240:c1486512-1486201 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51721 WP_000356397.1 NZ_CP010240:c1486200-1485910 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51721 NZ_CP010240:c1486200-1485910 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7