Detailed information of TA system TA051711

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 140909..141335
• Replicon: plasmid A
• Accession: NZ_CP010239
• Organism: Escherichia coli strain S50

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG72_RS26345
• Protein ID: WP_001312861.1
• Coordinates: 140909..141067 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	141111..141335 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG72_RS26300	135932..136159	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
RG72_RS26305	136296..136967	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
RG72_RS26310	137161..137544	-	384	WP_001354030.1	relaxosome protein TraM	-
RG72_RS26315	137867..138469	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
RG72_RS26320	138766..139587	-	822	WP_001234445.1	DUF945 domain-containing protein	-
RG72_RS26325	139698..139994	-	297	WP_001272251.1	hypothetical protein	-
RG72_RS28190	140294..140671	+	378	Protein_173	hypothetical protein	-
RG72_RS26345	140909..141067	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	141111..141335	-	225	NuclAT_0	-	Antitoxin
-	141111..141335	-	225	NuclAT_0	-	Antitoxin
-	141111..141335	-	225	NuclAT_0	-	Antitoxin
-	141111..141335	-	225	NuclAT_0	-	Antitoxin
RG72_RS28195	141147..141326	+	180	WP_001309233.1	hypothetical protein	-
RG72_RS26355	141347..142066	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
RG72_RS26360	142063..142497	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
RG72_RS26365	142566..144534	-	1969	Protein_178	ParB/RepB/Spo0J family partition protein	-
RG72_RS26370	144595..144828	-	234	WP_000006003.1	DUF905 family protein	-
RG72_RS26375	144886..145413	-	528	WP_073569710.1	single-stranded DNA-binding protein	-
RG72_RS26380	145629..146192	-	564	WP_001298559.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / blaDHA-1 / qnrB4	hlyC / hlyA / hlyB / hlyD / senB / pet	1..184614	184614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51711 WP_001312861.1 NZ_CP010239:c141067-140909 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51711 NZ_CP010239:c141067-140909 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT51711 NZ_CP010239:c141335-141111 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin