Detailed information of TA system TA051706

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4364..4785
• Replicon: plasmid A
• Accession: NZ_CP010239
• Organism: Escherichia coli strain S50

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: RG72_RS25320
• Protein ID: WP_001302184.1
• Coordinates: 4364..4522 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4587..4785 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG72_RS25280	637..1020	-	384	WP_021569028.1	relaxosome protein TraM	-
RG72_RS25290	1433..1943	+	511	Protein_2	transglycosylase SLT domain-containing protein	-
RG72_RS25295	2238..3059	-	822	WP_024239203.1	DUF945 domain-containing protein	-
RG72_RS25300	3178..3465	-	288	WP_021569031.1	hypothetical protein	-
RG72_RS28140	3490..3696	-	207	WP_000547965.1	hypothetical protein	-
RG72_RS25320	4364..4522	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	4587..4785	-	199	NuclAT_1	-	Antitoxin
-	4587..4785	-	199	NuclAT_1	-	Antitoxin
-	4587..4785	-	199	NuclAT_1	-	Antitoxin
-	4587..4785	-	199	NuclAT_1	-	Antitoxin
RG72_RS27835	4754..5522	-	769	Protein_7	plasmid SOS inhibition protein A	-
RG72_RS28365	5556..6125	-	570	Protein_8	ParB N-terminal domain-containing protein	-
RG72_RS25335	6191..6424	-	234	WP_024239188.1	DUF905 family protein	-
RG72_RS25340	6481..6933	-	453	WP_021569034.1	single-stranded DNA-binding protein	-
RG72_RS28145	7251..7761	+	511	Protein_11	hypothetical protein	-
RG72_RS25355	7731..7922	-	192	WP_001027495.1	hypothetical protein	-
RG72_RS25360	7919..8341	-	423	WP_024239105.1	DUF1380 family protein	-
RG72_RS25365	8388..8690	-	303	WP_122998873.1	antirestriction protein	-
RG72_RS28150	8994..9749	-	756	WP_053877346.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / blaDHA-1 / qnrB4	hlyC / hlyA / hlyB / hlyD / senB / pet	1..184614	184614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T51706 WP_001302184.1 NZ_CP010239:c4522-4364 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51706 NZ_CP010239:c4522-4364 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT51706 NZ_CP010239:c4785-4587 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin