Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 63087..63589 Replicon chromosome
Accession NZ_CP010238
Organism Escherichia coli strain S50

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG72_RS00430 Protein ID WP_000767829.1
Coordinates 63087..63341 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG72_RS00435 Protein ID WP_001259255.1
Coordinates 63338..63589 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG72_RS00405 58102..58329 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG72_RS00410 58343..59401 - 1059 WP_000492335.1 transport protein YeeE -
RG72_RS00415 59580..60938 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG72_RS27345 60928..60990 - 63 WP_010723108.1 membrane protein YoeI -
RG72_RS00420 61205..62134 - 930 WP_073569590.1 LysR family transcriptional regulator -
RG72_RS00425 62180..63004 - 825 WP_000754737.1 SDR family oxidoreductase -
RG72_RS00430 63087..63341 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG72_RS00435 63338..63589 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG72_RS00440 63872..63922 + 51 WP_001364200.1 his operon leader peptide -
RG72_RS00450 64068..64967 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG72_RS00455 64973..66277 + 1305 WP_001295420.1 histidinol dehydrogenase -
RG72_RS00460 66274..67344 + 1071 WP_001099221.1 histidinol-phosphate transaminase -
RG72_RS00465 67344..68411 + 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51684 WP_000767829.1 NZ_CP010238:c63341-63087 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51684 NZ_CP010238:c63341-63087 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51684 WP_001259255.1 NZ_CP010238:c63589-63338 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51684 NZ_CP010238:c63589-63338 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References