Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3719950..3720452 Replicon chromosome
Accession NZ_CP010237
Organism Escherichia coli strain S43

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG71_RS19300 Protein ID WP_000767829.1
Coordinates 3720198..3720452 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG71_RS19295 Protein ID WP_001259255.1
Coordinates 3719950..3720201 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG71_RS19265 3715128..3716195 - 1068 WP_023486671.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG71_RS19270 3716195..3717265 - 1071 WP_073528411.1 histidinol-phosphate transaminase -
RG71_RS19275 3717262..3718566 - 1305 WP_001317939.1 histidinol dehydrogenase -
RG71_RS19280 3718572..3719471 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG71_RS19290 3719617..3719667 - 51 WP_001364200.1 his operon leader peptide -
RG71_RS19295 3719950..3720201 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG71_RS19300 3720198..3720452 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG71_RS19305 3720535..3721359 + 825 WP_000754763.1 SDR family oxidoreductase -
RG71_RS19310 3721405..3722334 + 930 WP_000803366.1 LysR family transcriptional regulator -
RG71_RS27390 3722549..3722611 + 63 WP_010723108.1 membrane protein YoeI -
RG71_RS19315 3722601..3723959 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG71_RS19320 3724137..3725195 + 1059 WP_023486673.1 transport protein YeeE -
RG71_RS19325 3725209..3725436 + 228 WP_023486674.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51672 WP_000767829.1 NZ_CP010237:3720198-3720452 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51672 NZ_CP010237:3720198-3720452 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51672 WP_001259255.1 NZ_CP010237:3719950-3720201 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51672 NZ_CP010237:3719950-3720201 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References