Detailed information of TA system TA051663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 793716..794410
• Replicon: chromosome
• Accession: NZ_CP010237
• Organism: Escherichia coli strain S43

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG71_RS04360
• Protein ID: WP_001263493.1
• Coordinates: 793716..794114 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: RG71_RS04365
• Protein ID: WP_073528149.1
• Coordinates: 794117..794410 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG71_RS04330	788716..789960	-	1245	WP_000189541.1	esterase FrsA	-
-	789376..789456	-	81	NuclAT_8	-	-
-	789376..789456	-	81	NuclAT_8	-	-
-	789376..789456	-	81	NuclAT_8	-	-
-	789376..789456	-	81	NuclAT_8	-	-
RG71_RS04335	790052..790510	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG71_RS04340	790771..792228	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG71_RS04345	792285..792806	-	522	Protein_774	peptide chain release factor H	-
RG71_RS04350	792805..793008	-	204	Protein_775	RNA ligase RtcB family protein	-
RG71_RS04355	793254..793706	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG71_RS04360	793716..794114	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG71_RS04365	794117..794410	-	294	WP_073528149.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG71_RS04370	794462..795517	-	1056	WP_001226164.1	DNA polymerase IV	-
RG71_RS04375	795588..796373	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
RG71_RS04380	796345..798057	+	1713	Protein_781	flagellar biosynthesis protein FlhA	-
RG71_RS04395	798273..798770	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssM / tssA / hcp1/tssD1	760064..878595	118531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T51663 WP_001263493.1 NZ_CP010237:c794114-793716 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51663 NZ_CP010237:c794114-793716 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11232.82 Da
• Isoelectric Point: 7.0158

>AT51663 WP_073528149.1 NZ_CP010237:c794410-794117 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNNMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51663 NZ_CP010237:c794410-794117 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATAATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8