51651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1979486..1979988 Replicon chromosome
Accession NZ_CP010236
Organism Escherichia coli strain S42

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG70_RS10535 Protein ID WP_000767829.1
Coordinates 1979486..1979740 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG70_RS10540 Protein ID WP_001259255.1
Coordinates 1979737..1979988 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG70_RS10510 1974501..1974728 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG70_RS10515 1974742..1975800 - 1059 WP_000492346.1 transport protein YeeE -
RG70_RS10520 1975979..1977337 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG70_RS25660 1977327..1977389 - 63 WP_010723108.1 membrane protein YoeI -
RG70_RS10525 1977604..1978533 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG70_RS10530 1978579..1979403 - 825 WP_021562277.1 SDR family oxidoreductase -
RG70_RS10535 1979486..1979740 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG70_RS10540 1979737..1979988 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG70_RS10545 1980271..1980321 + 51 WP_001364200.1 his operon leader peptide -
RG70_RS10555 1980467..1981366 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG70_RS10560 1981372..1982676 + 1305 WP_001570037.1 histidinol dehydrogenase -
RG70_RS10565 1982673..1983743 + 1071 WP_000108963.1 histidinol-phosphate transaminase -
RG70_RS10570 1983743..1984810 + 1068 WP_029397756.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51651 WP_000767829.1 NZ_CP010236:c1979740-1979486 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51651 NZ_CP010236:c1979740-1979486 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51651 WP_001259255.1 NZ_CP010236:c1979988-1979737 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51651 NZ_CP010236:c1979988-1979737 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References