Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3953783..3954324 Replicon chromosome
Accession NZ_CP010235
Organism Escherichia coli strain S40

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag RG69_RS20695 Protein ID WP_000615982.1
Coordinates 3954046..3954324 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG69_RS20690 Protein ID WP_000729704.1
Coordinates 3953783..3954043 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG69_RS20665 3949566..3950351 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
RG69_RS20670 3950323..3952035 + 1713 Protein_3759 flagellar biosynthesis protein FlhA -
RG69_RS20675 3952140..3952418 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RG69_RS20680 3952411..3952767 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RG69_RS20685 3952824..3953597 - 774 WP_000543899.1 C40 family peptidase -
RG69_RS20690 3953783..3954043 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG69_RS20695 3954046..3954324 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG69_RS20700 3954480..3955220 + 741 WP_001225679.1 murein L,D-transpeptidase -
RG69_RS20705 3955191..3955958 - 768 WP_000333380.1 class II glutamine amidotransferase -
RG69_RS20715 3956164..3956742 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T51630 WP_000615982.1 NZ_CP010235:3954046-3954324 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T51630 NZ_CP010235:3954046-3954324 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT51630 WP_000729704.1 NZ_CP010235:3953783-3954043 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT51630 NZ_CP010235:3953783-3954043 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References