Detailed information of TA system TA051600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4215120..4215814
• Replicon: chromosome
• Accession: NZ_CP010231
• Organism: Escherichia coli strain S30

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG68_RS22180
• Protein ID: WP_001263493.1
• Coordinates: 4215416..4215814 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG68_RS22175
• Protein ID: WP_000554757.1
• Coordinates: 4215120..4215413 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG68_RS22150	4210752..4211249	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
RG68_RS22160	4211473..4213185	-	1713	Protein_3946	flagellar biosynthesis protein FlhA	-
RG68_RS22165	4213157..4213942	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
RG68_RS22170	4214013..4215068	+	1056	WP_001226164.1	DNA polymerase IV	-
RG68_RS22175	4215120..4215413	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG68_RS22180	4215416..4215814	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG68_RS22185	4215824..4216276	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG68_RS22190	4216522..4216725	+	204	Protein_3952	RNA ligase RtcB family protein	-
RG68_RS22195	4216724..4217245	+	522	Protein_3953	peptide chain release factor H	-
RG68_RS22200	4217302..4218759	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
RG68_RS22205	4219020..4219478	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG68_RS22210	4219570..4220814	+	1245	WP_000189541.1	esterase FrsA	-
-	4220074..4220154	+	81	NuclAT_11	-	-
-	4220074..4220154	+	81	NuclAT_11	-	-
-	4220074..4220154	+	81	NuclAT_11	-	-
-	4220074..4220154	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T51600 WP_001263493.1 NZ_CP010231:4215416-4215814 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51600 NZ_CP010231:4215416-4215814 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51600 WP_000554757.1 NZ_CP010231:4215120-4215413 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51600 NZ_CP010231:4215120-4215413 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6