Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1399681..1400183 Replicon chromosome
Accession NZ_CP010231
Organism Escherichia coli strain S30

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG68_RS07475 Protein ID WP_000767829.1
Coordinates 1399681..1399935 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG68_RS07480 Protein ID WP_001259255.1
Coordinates 1399932..1400183 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG68_RS07450 1394696..1394923 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG68_RS07455 1394937..1395995 - 1059 WP_000492315.1 transport protein YeeE -
RG68_RS07460 1396174..1397532 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG68_RS28345 1397522..1397584 - 63 WP_010723108.1 membrane protein YoeI -
RG68_RS07465 1397799..1398728 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG68_RS07470 1398774..1399598 - 825 WP_000754763.1 SDR family oxidoreductase -
RG68_RS07475 1399681..1399935 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG68_RS07480 1399932..1400183 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG68_RS07485 1400466..1400516 + 51 WP_001364200.1 his operon leader peptide -
RG68_RS07495 1400662..1401561 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG68_RS07500 1401567..1402871 + 1305 WP_073546517.1 histidinol dehydrogenase -
RG68_RS07505 1402868..1403938 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG68_RS07510 1403938..1405005 + 1068 WP_063090919.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51591 WP_000767829.1 NZ_CP010231:c1399935-1399681 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51591 NZ_CP010231:c1399935-1399681 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51591 WP_001259255.1 NZ_CP010231:c1400183-1399932 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51591 NZ_CP010231:c1400183-1399932 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References