Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4397528..4398069 Replicon chromosome
Accession NZ_CP010230
Organism Escherichia coli strain S21

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag RG67_RS22595 Protein ID WP_000615982.1
Coordinates 4397528..4397806 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG67_RS22600 Protein ID WP_000729704.1
Coordinates 4397809..4398069 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG67_RS22575 4395110..4395688 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
RG67_RS22585 4395894..4396661 + 768 WP_000333380.1 class II glutamine amidotransferase -
RG67_RS22590 4396632..4397372 - 741 WP_001225679.1 murein L,D-transpeptidase -
RG67_RS22595 4397528..4397806 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG67_RS22600 4397809..4398069 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG67_RS22605 4398255..4399028 + 774 WP_000543899.1 C40 family peptidase -
RG67_RS22610 4399085..4399441 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RG67_RS22615 4399434..4399712 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RG67_RS22620 4399817..4401529 - 1713 Protein_4079 flagellar biosynthesis protein FlhA -
RG67_RS22625 4401501..4402286 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4389287..4399712 10425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T51578 WP_000615982.1 NZ_CP010230:c4397806-4397528 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T51578 NZ_CP010230:c4397806-4397528 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT51578 WP_000729704.1 NZ_CP010230:c4398069-4397809 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT51578 NZ_CP010230:c4398069-4397809 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References