Detailed information of TA system TA051575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3569309..3569911
• Replicon: chromosome
• Accession: NZ_CP010230
• Organism: Escherichia coli strain S21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG67_RS18490
• Protein ID: WP_000897305.1
• Coordinates: 3569309..3569620 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG67_RS18495
• Protein ID: WP_000356397.1
• Coordinates: 3569621..3569911 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG67_RS18460	3564339..3565124	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
RG67_RS18465	3565223..3565822	+	600	WP_001315111.1	glucose-1-phosphatase	-
RG67_RS18470	3565816..3566688	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG67_RS18475	3566685..3567122	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG67_RS18480	3567167..3568108	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RG67_RS18485	3568172..3569080	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG67_RS18490	3569309..3569620	+	312	WP_000897305.1	hypothetical protein	Toxin
RG67_RS18495	3569621..3569911	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG67_RS18505	3570516..3570734	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG67_RS18510	3570953..3571195	+	243	WP_001086388.1	hypothetical protein	-
RG67_RS18520	3571525..3572454	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
RG67_RS18525	3572451..3573086	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG67_RS18530	3573083..3573985	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51575 WP_000897305.1 NZ_CP010230:3569309-3569620 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51575 NZ_CP010230:3569309-3569620 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51575 WP_000356397.1 NZ_CP010230:3569621-3569911 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51575 NZ_CP010230:3569621-3569911 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7