Detailed information of TA system TA051556

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 854827..855198
• Replicon: chromosome
• Accession: NZ_CP010230
• Organism: Escherichia coli strain S21

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG67_RS04550
• Protein ID: WP_001317028.1
• Coordinates: 855004..855198 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	854827..855005 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG67_RS04525	850782..852155	+	1374	WP_000123763.1	ATP-dependent RNA helicase DbpA	-
RG67_RS04530	852284..853219	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG67_RS04535	853271..854506	-	1236	WP_073508607.1	site-specific integrase	-
RG67_RS04540	854508..854723	-	216	WP_000079604.1	excisionase XisR	-
RG67_RS04545	854802..855011	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	854827..855005	+	179	NuclAT_0	-	Antitoxin
-	854827..855005	+	179	NuclAT_0	-	Antitoxin
-	854827..855005	+	179	NuclAT_0	-	Antitoxin
-	854827..855005	+	179	NuclAT_0	-	Antitoxin
RG67_RS04550	855004..855198	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG67_RS04555	855255..856064	-	810	WP_000166319.1	recombination protein RecT	-
RG67_RS04560	856057..858648	-	2592	WP_000105142.1	exodeoxyribonuclease VIII	-
RG67_RS04565	858750..859025	-	276	WP_000632297.1	protein RacC	-
RG67_RS04570	859100..859270	-	171	WP_001352098.1	conserved protein, Rac prophage	-
RG67_RS04575	859270..859491	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	844526..905572	61046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T51556 WP_001317028.1 NZ_CP010230:c855198-855004 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T51556 NZ_CP010230:c855198-855004 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT51556 NZ_CP010230:854827-855005 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin