Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3494426..3494928 Replicon chromosome
Accession NZ_CP010229
Organism Escherichia coli strain S10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG66_RS17830 Protein ID WP_000767829.1
Coordinates 3494674..3494928 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG66_RS17825 Protein ID WP_001259255.1
Coordinates 3494426..3494677 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG66_RS17795 3489604..3490671 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG66_RS17800 3490671..3491741 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG66_RS17805 3491738..3493042 - 1305 WP_073535727.1 histidinol dehydrogenase -
RG66_RS17810 3493048..3493947 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG66_RS17820 3494093..3494143 - 51 WP_001364200.1 his operon leader peptide -
RG66_RS17825 3494426..3494677 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG66_RS17830 3494674..3494928 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG66_RS17835 3495011..3495835 + 825 WP_000754737.1 SDR family oxidoreductase -
RG66_RS17840 3495881..3496810 + 930 WP_032283327.1 LysR family transcriptional regulator -
RG66_RS25980 3497025..3497087 + 63 WP_010723108.1 membrane protein YoeI -
RG66_RS17845 3497077..3498435 + 1359 WP_024198437.1 putrescine/proton symporter PlaP -
RG66_RS17850 3498614..3499672 + 1059 WP_040072176.1 transport protein YeeE -
RG66_RS17855 3499686..3499913 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51546 WP_000767829.1 NZ_CP010229:3494674-3494928 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51546 NZ_CP010229:3494674-3494928 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51546 WP_001259255.1 NZ_CP010229:3494426-3494677 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51546 NZ_CP010229:3494426-3494677 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References