Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1779030..1779556 Replicon chromosome
Accession NZ_CP010228
Organism Escherichia coli strain S3

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag RG65_RS09365 Protein ID WP_000323025.1
Coordinates 1779269..1779556 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag RG65_RS09360 Protein ID WP_000534858.1
Coordinates 1779030..1779269 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG65_RS09310 1774536..1774727 - 192 WP_001083297.1 lysis protein YdfD -
RG65_RS09315 1774724..1774912 - 189 WP_000854559.1 cell division inhibition protein DicB -
RG65_RS09340 1776790..1777728 + 939 WP_213056067.1 ISNCY family transposase -
RG65_RS09345 1778165..1778497 - 333 WP_001301033.1 protein FlxA -
RG65_RS09355 1778700..1779005 - 306 WP_001326990.1 hypothetical protein -
RG65_RS09360 1779030..1779269 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
RG65_RS09365 1779269..1779556 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
RG65_RS09370 1779628..1779783 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
RG65_RS09375 1780000..1780251 + 252 WP_000980994.1 hypothetical protein -
RG65_RS09380 1780318..1780596 + 279 WP_012304870.1 hypothetical protein -
RG65_RS09385 1780598..1781647 + 1050 WP_001265198.1 DUF968 domain-containing protein -
RG65_RS09390 1781661..1782413 + 753 WP_073507359.1 antitermination protein -
RG65_RS24860 1782691..1782780 - 90 WP_120795389.1 hypothetical protein -
RG65_RS09395 1782835..1783047 - 213 WP_000087756.1 cold shock-like protein CspF -
RG65_RS09400 1783348..1783563 + 216 WP_000066484.1 cold shock-like protein CspB -
RG65_RS25380 1783927..1784097 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
RG65_RS09405 1784317..1784532 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1772384..1794137 21753


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T51530 WP_000323025.1 NZ_CP010228:1779269-1779556 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T51530 NZ_CP010228:1779269-1779556 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT51530 WP_000534858.1 NZ_CP010228:1779030-1779269 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT51530 NZ_CP010228:1779030-1779269 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References