Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1333817..1334319 Replicon chromosome
Accession NZ_CP010228
Organism Escherichia coli strain S3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG65_RS06885 Protein ID WP_000767829.1
Coordinates 1334065..1334319 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG65_RS06880 Protein ID WP_001259255.1
Coordinates 1333817..1334068 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG65_RS06850 1328995..1330062 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG65_RS06855 1330062..1331132 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
RG65_RS06860 1331129..1332433 - 1305 WP_001385864.1 histidinol dehydrogenase -
RG65_RS06865 1332439..1333338 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
RG65_RS06875 1333484..1333534 - 51 WP_001364200.1 his operon leader peptide -
RG65_RS06880 1333817..1334068 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG65_RS06885 1334065..1334319 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG65_RS06890 1334402..1335226 + 825 WP_000754758.1 SDR family oxidoreductase -
RG65_RS06895 1335272..1336201 + 930 WP_000803351.1 LysR family transcriptional regulator -
RG65_RS24810 1336416..1336478 + 63 WP_010723108.1 membrane protein YoeI -
RG65_RS06900 1336468..1337826 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG65_RS06905 1338005..1339063 + 1059 WP_073507316.1 transport protein YeeE -
RG65_RS06910 1339077..1339304 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51521 WP_000767829.1 NZ_CP010228:1334065-1334319 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51521 NZ_CP010228:1334065-1334319 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51521 WP_001259255.1 NZ_CP010228:1333817-1334068 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51521 NZ_CP010228:1333817-1334068 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References