Detailed information of TA system TA051515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3204529..3205223
• Replicon: chromosome
• Accession: NZ_CP010226
• Organism: Escherichia coli strain S1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: RG64_RS17595
• Protein ID: WP_001263493.1
• Coordinates: 3204529..3204927 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RG64_RS17600
• Protein ID: WP_000554757.1
• Coordinates: 3204930..3205223 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG64_RS17565	3199529..3200773	-	1245	WP_000189541.1	esterase FrsA	-
-	3200189..3200269	-	81	NuclAT_11	-	-
-	3200189..3200269	-	81	NuclAT_11	-	-
-	3200189..3200269	-	81	NuclAT_11	-	-
-	3200189..3200269	-	81	NuclAT_11	-	-
RG64_RS17570	3200865..3201323	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RG64_RS17575	3201584..3203041	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
RG64_RS17580	3203098..3203619	-	522	Protein_3105	peptide chain release factor H	-
RG64_RS17585	3203618..3203821	-	204	Protein_3106	RNA ligase RtcB family protein	-
RG64_RS17590	3204067..3204519	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
RG64_RS17595	3204529..3204927	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RG64_RS17600	3204930..3205223	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RG64_RS17605	3205275..3206330	-	1056	WP_001226164.1	DNA polymerase IV	-
RG64_RS17610	3206401..3207186	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
RG64_RS17615	3207158..3208870	+	1713	Protein_3112	flagellar biosynthesis protein FlhA	-
RG64_RS17630	3209086..3209583	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3155188..3234821	79633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T51515 WP_001263493.1 NZ_CP010226:c3204927-3204529 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T51515 NZ_CP010226:c3204927-3204529 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT51515 WP_000554757.1 NZ_CP010226:c3205223-3204930 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT51515 NZ_CP010226:c3205223-3204930 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6