Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1312301..1312803 Replicon chromosome
Accession NZ_CP010226
Organism Escherichia coli strain S1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG64_RS06925 Protein ID WP_000767829.1
Coordinates 1312549..1312803 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG64_RS06920 Protein ID WP_001259255.1
Coordinates 1312301..1312552 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG64_RS06890 1307479..1308546 - 1068 WP_029397756.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG64_RS06895 1308546..1309616 - 1071 WP_000108963.1 histidinol-phosphate transaminase -
RG64_RS06900 1309613..1310917 - 1305 WP_032177449.1 histidinol dehydrogenase -
RG64_RS06905 1310923..1311822 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG64_RS06915 1311968..1312018 - 51 WP_001364200.1 his operon leader peptide -
RG64_RS06920 1312301..1312552 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG64_RS06925 1312549..1312803 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG64_RS06930 1312886..1313710 + 825 WP_000754752.1 SDR family oxidoreductase -
RG64_RS06935 1313756..1314685 + 930 WP_032177447.1 LysR family transcriptional regulator -
RG64_RS26550 1314900..1314962 + 63 WP_010723108.1 membrane protein YoeI -
RG64_RS06940 1314952..1316310 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG64_RS25855 1316420..1317103 + 684 Protein_1233 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1316420..1316695 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51500 WP_000767829.1 NZ_CP010226:1312549-1312803 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51500 NZ_CP010226:1312549-1312803 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51500 WP_001259255.1 NZ_CP010226:1312301-1312552 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51500 NZ_CP010226:1312301-1312552 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References