51500

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1312301..1312803 Replicon chromosome
Accession NZ_CP010226
Organism Escherichia coli strain S1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG64_RS06925 Protein ID WP_000767829.1
Coordinates 1312549..1312803 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG64_RS06920 Protein ID WP_001259255.1
Coordinates 1312301..1312552 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG64_RS06890 1307479..1308546 - 1068 WP_029397756.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
RG64_RS06895 1308546..1309616 - 1071 WP_000108963.1 histidinol-phosphate transaminase -
RG64_RS06900 1309613..1310917 - 1305 WP_032177449.1 histidinol dehydrogenase -
RG64_RS06905 1310923..1311822 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG64_RS06915 1311968..1312018 - 51 WP_001364200.1 his operon leader peptide -
RG64_RS06920 1312301..1312552 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG64_RS06925 1312549..1312803 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG64_RS06930 1312886..1313710 + 825 WP_000754752.1 SDR family oxidoreductase -
RG64_RS06935 1313756..1314685 + 930 WP_032177447.1 LysR family transcriptional regulator -
RG64_RS26550 1314900..1314962 + 63 WP_010723108.1 membrane protein YoeI -
RG64_RS06940 1314952..1316310 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG64_RS25855 1316420..1317103 + 684 Protein_1233 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1316420..1316695 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51500 WP_000767829.1 NZ_CP010226:1312549-1312803 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51500 NZ_CP010226:1312549-1312803 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51500 WP_001259255.1 NZ_CP010226:1312301-1312552 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51500 NZ_CP010226:1312301-1312552 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References