Detailed information of TA system TA051485

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14827..15218
• Replicon: plasmid A
• Accession: NZ_CP010222
• Organism: Escherichia coli strain M19

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG63_RS26630
• Protein ID: WP_073519970.1
• Coordinates: 15057..15218 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14827..15004 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG63_RS29190	9911..10132	-	222	WP_154813206.1	hypothetical protein	-
RG63_RS29575	10197..10346	-	150	WP_157920373.1	hypothetical protein	-
RG63_RS26595	10417..10974	+	558	WP_073519975.1	single-stranded DNA-binding protein	-
RG63_RS26600	11026..11268	+	243	WP_073519974.1	DUF905 domain-containing protein	-
RG63_RS26605	11338..13335	+	1998	WP_073519973.1	ParB/RepB/Spo0J family partition protein	-
RG63_RS26610	13376..13810	+	435	WP_097455818.1	conjugation system SOS inhibitor PsiB	-
RG63_RS26615	13807..14536	+	730	Protein_20	plasmid SOS inhibition protein A	-
RG63_RS26620	14533..14847	+	315	WP_073519971.1	theronine dehydrogenase	-
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
RG63_RS26630	15057..15218	+	162	WP_073519970.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG63_RS28510	15392..15766	+	375	WP_171769349.1	hypothetical protein	-
RG63_RS29200	15951..16362	-	412	Protein_24	hypothetical protein	-
RG63_RS26650	16659..17003	+	345	WP_073519969.1	hypothetical protein	-
RG63_RS26655	17147..17968	+	822	WP_073519968.1	DUF945 domain-containing protein	-
RG63_RS26660	18264..18773	-	510	WP_073519994.1	transglycosylase SLT domain-containing protein	-
RG63_RS26665	19197..19583	+	387	WP_073519967.1	relaxosome protein TraM	-
RG63_RS26670	19772..20194	+	423	WP_073519966.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / faeC / faeD / faeE / faeF / faeH / faeI	1..146552	146552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5992.17 Da
• Isoelectric Point: 8.7840

>T51485 WP_073519970.1 NZ_CP010222:15057-15218 [Escherichia coli]
MKLPRNALIWCVLIVCITLLIFTGLTRNRLCEVRLKDGEREVAASLAYESNGK

Nucleotide

• Length: 162 bp

>T51485 NZ_CP010222:15057-15218 [Escherichia coli]
ATGAAACTGCCGCGAAACGCCCTTATCTGGTGCGTATTGATCGTGTGTATCACACTCTTGATATTCACAGGTCTGACCCG
GAATCGCTTATGTGAAGTCCGGCTGAAGGACGGAGAAAGGGAGGTCGCGGCAAGCCTGGCTTACGAATCCAACGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 178 bp

>AT51485 NZ_CP010222:14827-15004 [Escherichia coli]
GATTATTTTCGGGGAAAGTGATGGCAGTGGTCAGCGTCGGTCCGTATAGGGTATGATGTGCCCGGCGCTTGAGGCTTTCT
GCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTATTCATTAACCCA
CGAGGCCCCCTGATGTCT

Structures

Antitoxin