Detailed information of TA system TA051479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3442968..3443570
• Replicon: chromosome
• Accession: NZ_CP010221
• Organism: Escherichia coli strain M19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG63_RS17915
• Protein ID: WP_000897305.1
• Coordinates: 3442968..3443279 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG63_RS17920
• Protein ID: WP_000356397.1
• Coordinates: 3443280..3443570 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG63_RS17890	3438882..3439481	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG63_RS17895	3439475..3440347	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG63_RS17900	3440344..3440781	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG63_RS17905	3440826..3441767	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
RG63_RS17910	3441831..3442739	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG63_RS17915	3442968..3443279	+	312	WP_000897305.1	hypothetical protein	Toxin
RG63_RS17920	3443280..3443570	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG63_RS17930	3444175..3444393	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG63_RS17935	3444612..3444854	+	243	WP_001086388.1	hypothetical protein	-
RG63_RS28315	3445083..3446064	-	982	Protein_3240	IS110-like element IS621 family transposase	-
RG63_RS17960	3446463..3447392	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG63_RS17965	3447389..3448024	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51479 WP_000897305.1 NZ_CP010221:3442968-3443279 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51479 NZ_CP010221:3442968-3443279 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51479 WP_000356397.1 NZ_CP010221:3443280-3443570 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51479 NZ_CP010221:3443280-3443570 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7