Detailed information of TA system TA051456

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 5076867..5077286
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: RG62_RS26650
• Protein ID: WP_000775238.1
• Coordinates: 5076867..5077028 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	5077074..5077286 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS26600	5072673..5073056	-	384	WP_046201845.1	relaxosome protein TraM	-
RG62_RS26610	5073379..5073981	+	603	WP_077787755.1	transglycosylase SLT domain-containing protein	-
RG62_RS26615	5074277..5075098	-	822	WP_046201784.1	DUF945 domain-containing protein	-
RG62_RS26620	5075217..5075504	-	288	WP_046201785.1	hypothetical protein	-
RG62_RS26630	5075660..5075962	-	303	WP_046201786.1	hypothetical protein	-
RG62_RS28240	5075986..5076192	-	207	WP_046201787.1	hypothetical protein	-
RG62_RS28495	5076262..5076550	+	289	Protein_4793	hypothetical protein	-
RG62_RS26650	5076867..5077028	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	5077074..5077286	-	213	NuclAT_1	-	Antitoxin
-	5077074..5077286	-	213	NuclAT_1	-	Antitoxin
-	5077074..5077286	-	213	NuclAT_1	-	Antitoxin
-	5077074..5077286	-	213	NuclAT_1	-	Antitoxin
RG62_RS26660	5077235..5077549	-	315	WP_000872077.1	hypothetical protein	-
RG62_RS26665	5077546..5078265	-	720	WP_046201788.1	plasmid SOS inhibition protein A	-
RG62_RS26670	5078262..5078696	-	435	WP_046201789.1	conjugation system SOS inhibitor PsiB	-
RG62_RS26675	5078751..5080709	-	1959	WP_046201790.1	ParB/RepB/Spo0J family partition protein	-
RG62_RS26680	5080770..5081003	-	234	WP_046201791.1	DUF905 family protein	-
RG62_RS26685	5081066..5081599	-	534	WP_046201792.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pic	4835798..5080594	244796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T51456 WP_000775238.1 NZ_CP010219:c5077028-5076867 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T51456 NZ_CP010219:c5077028-5076867 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACTCTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 213 bp

>AT51456 NZ_CP010219:c5077286-5077074 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin