Detailed information of TA system TA051447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2960006..2960608
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG62_RS15475
• Protein ID: WP_000897305.1
• Coordinates: 2960006..2960317 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG62_RS15480
• Protein ID: WP_073527692.1
• Coordinates: 2960318..2960608 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS15450	2955920..2956519	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG62_RS15455	2956513..2957385	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG62_RS15460	2957382..2957819	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG62_RS15465	2957864..2958805	+	942	WP_001395735.1	fatty acid biosynthesis protein FabY	-
RG62_RS15470	2958869..2959777	-	909	WP_001162704.1	alpha/beta hydrolase	-
RG62_RS15475	2960006..2960317	+	312	WP_000897305.1	hypothetical protein	Toxin
RG62_RS15480	2960318..2960608	+	291	WP_073527692.1	helix-turn-helix domain-containing protein	Antitoxin
RG62_RS15490	2961213..2961431	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
RG62_RS15495	2961650..2961892	+	243	WP_001086388.1	hypothetical protein	-
RG62_RS15505	2962222..2963151	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG62_RS15510	2963148..2963783	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG62_RS15515	2963780..2964682	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51447 WP_000897305.1 NZ_CP010219:2960006-2960317 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51447 NZ_CP010219:2960006-2960317 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10850.42 Da
• Isoelectric Point: 10.8907

>AT51447 WP_073527692.1 NZ_CP010219:2960318-2960608 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDTQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51447 NZ_CP010219:2960318-2960608 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATACGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7