Detailed information of TA system TA051446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2171394..2172193
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: RG62_RS11355
• Protein ID: WP_000347253.1
• Coordinates: 2171729..2172193 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: RG62_RS11350
• Protein ID: WP_024251361.1
• Coordinates: 2171394..2171729 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS11335	2167179..2167949	-	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-
RG62_RS11340	2167965..2169299	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
RG62_RS11345	2169674..2171245	+	1572	WP_001273766.1	galactarate dehydratase	-
RG62_RS11350	2171394..2171729	+	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
RG62_RS11355	2171729..2172193	+	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
RG62_RS11360	2172248..2173057	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
RG62_RS11370	2173306..2174586	+	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
RG62_RS11375	2174609..2175082	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
RG62_RS11380	2175093..2175872	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
RG62_RS11385	2175862..2176740	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
RG62_RS11390	2176758..2177192	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2160519..2172193	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T51446 WP_000347253.1 NZ_CP010219:2171729-2172193 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T51446 NZ_CP010219:2171729-2172193 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT51446 WP_024251361.1 NZ_CP010219:2171394-2171729 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT51446 NZ_CP010219:2171394-2171729 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA