Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 901671..902173 Replicon chromosome
Accession NZ_CP010219
Organism Escherichia coli strain M18

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag RG62_RS04895 Protein ID WP_000767829.1
Coordinates 901671..901925 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag RG62_RS04900 Protein ID WP_001259255.1
Coordinates 901922..902173 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG62_RS04870 896686..896913 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
RG62_RS04875 896927..897985 - 1059 WP_000492346.1 transport protein YeeE -
RG62_RS04880 898164..899522 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
RG62_RS27760 899512..899574 - 63 WP_010723108.1 membrane protein YoeI -
RG62_RS04885 899789..900718 - 930 WP_000803351.1 LysR family transcriptional regulator -
RG62_RS04890 900764..901588 - 825 WP_000754758.1 SDR family oxidoreductase -
RG62_RS04895 901671..901925 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
RG62_RS04900 901922..902173 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
RG62_RS04905 902456..902506 + 51 WP_001364200.1 his operon leader peptide -
RG62_RS04915 902652..903551 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
RG62_RS04920 903557..904861 + 1305 WP_001542955.1 histidinol dehydrogenase -
RG62_RS04925 904858..905928 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
RG62_RS04930 905928..906995 + 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T51441 WP_000767829.1 NZ_CP010219:c901925-901671 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T51441 NZ_CP010219:c901925-901671 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT51441 WP_001259255.1 NZ_CP010219:c902173-901922 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT51441 NZ_CP010219:c902173-901922 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References