Detailed information of TA system TA051427

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 179629..179988
• Replicon: chromosome
• Accession: NZ_CP010219
• Organism: Escherichia coli strain M18

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG62_RS00965
• Protein ID: WP_001317028.1
• Coordinates: 179794..179988 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	179629..179795 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG62_RS00940	175584..176957	+	1374	WP_000123739.1	ATP-dependent RNA helicase DbpA	-
RG62_RS00945	177086..178021	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG62_RS00950	178073..179308	-	1236	WP_000040852.1	site-specific integrase	-
RG62_RS00955	179310..179525	-	216	WP_000079604.1	excisionase XisR	-
RG62_RS00960	179604..179801	-	198	WP_024170988.1	DUF1187 family protein	-
-	179629..179795	+	167	NuclAT_2	-	Antitoxin
-	179629..179795	+	167	NuclAT_2	-	Antitoxin
-	179629..179795	+	167	NuclAT_2	-	Antitoxin
-	179629..179795	+	167	NuclAT_2	-	Antitoxin
RG62_RS00965	179794..179988	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG62_RS00970	180045..180854	-	810	WP_000166319.1	recombination protein RecT	-
RG62_RS00975	180847..183447	-	2601	WP_000105136.1	exodeoxyribonuclease VIII	-
RG62_RS00980	183549..183824	-	276	WP_024251429.1	hypothetical protein	-
RG62_RS00985	183899..184069	-	171	WP_001352098.1	conserved protein, Rac prophage	-
RG62_RS00990	184069..184290	-	222	WP_000560224.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	178073..228507	50434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T51427 WP_001317028.1 NZ_CP010219:c179988-179794 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T51427 NZ_CP010219:c179988-179794 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 167 bp

>AT51427 NZ_CP010219:179629-179795 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin