Detailed information of TA system TA051421

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31910..32336
• Replicon: plasmid B
• Accession: NZ_CP010215
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG61_RS27655
• Protein ID: WP_001312861.1
• Coordinates: 31910..32068 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32112..32336 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS27605	26962..27189	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
RG61_RS27610	27283..27969	-	687	WP_073520017.1	PAS domain-containing protein	-
RG61_RS27615	28160..28543	-	384	WP_000124981.1	relaxosome protein TraM	-
RG61_RS27625	28820..29467	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
RG61_RS27630	29764..30585	-	822	WP_073520018.1	DUF945 domain-containing protein	-
RG61_RS27635	30703..30990	-	288	WP_000107535.1	hypothetical protein	-
RG61_RS27640	31014..31220	-	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG61_RS28555	31222..31410	-	189	WP_032141200.1	hypothetical protein	-
RG61_RS27655	31910..32068	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	32112..32336	-	225	NuclAT_0	-	Antitoxin
-	32112..32336	-	225	NuclAT_0	-	Antitoxin
-	32112..32336	-	225	NuclAT_0	-	Antitoxin
-	32112..32336	-	225	NuclAT_0	-	Antitoxin
RG61_RS27665	32348..33067	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
RG61_RS27670	33064..33498	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
RG61_RS27675	33553..35511	-	1959	WP_073520020.1	ParB/RepB/Spo0J family partition protein	-
RG61_RS27680	35576..35809	-	234	WP_001519997.1	DUF905 family protein	-
RG61_RS27685	35865..36404	-	540	WP_009426937.1	single-stranded DNA-binding protein	-
RG61_RS27690	36430..36636	-	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG61_RS27695	36638..36826	-	189	WP_032141200.1	hypothetical protein	-
RG61_RS29270	36749..36973	+	225	WP_013279293.1	hypothetical protein	-
RG61_RS27700	36877..37104	-	228	WP_071594011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61058	61058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51421 WP_001312861.1 NZ_CP010215:c32068-31910 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51421 NZ_CP010215:c32068-31910 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT51421 NZ_CP010215:c32336-32112 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin