Detailed information of TA system TA051417

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119445..119836
• Replicon: plasmid A
• Accession: NZ_CP010214
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG61_RS27305
• Protein ID: WP_073519970.1
• Coordinates: 119445..119606 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	119659..119836 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS27270	114469..114891	-	423	WP_073519966.1	PAS domain-containing protein	-
RG61_RS27275	115080..115466	-	387	WP_073519967.1	relaxosome protein TraM	-
RG61_RS27280	115890..116399	+	510	WP_073519994.1	transglycosylase SLT domain-containing protein	-
RG61_RS27285	116695..117516	-	822	WP_073519968.1	DUF945 domain-containing protein	-
RG61_RS27290	117660..118004	-	345	WP_073519969.1	hypothetical protein	-
RG61_RS27295	117994..118123	-	130	Protein_145	single-stranded DNA-binding protein	-
RG61_RS29240	118055..118264	+	210	Protein_146	hypothetical protein	-
RG61_RS29245	118301..118712	+	412	Protein_147	hypothetical protein	-
RG61_RS29250	118660..118884	-	225	WP_141221904.1	hypothetical protein	-
RG61_RS28545	118897..119337	-	441	WP_149025694.1	hypothetical protein	-
RG61_RS27305	119445..119606	-	162	WP_073519970.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	119659..119836	-	178	NuclAT_0	-	Antitoxin
-	119659..119836	-	178	NuclAT_0	-	Antitoxin
-	119659..119836	-	178	NuclAT_0	-	Antitoxin
-	119659..119836	-	178	NuclAT_0	-	Antitoxin
RG61_RS27310	119684..119835	+	152	Protein_151	protein sok	-
RG61_RS27315	119816..120130	-	315	WP_073519971.1	theronine dehydrogenase	-
RG61_RS27320	120127..120856	-	730	Protein_153	plasmid SOS inhibition protein A	-
RG61_RS27325	120853..121287	-	435	WP_097455818.1	conjugation system SOS inhibitor PsiB	-
RG61_RS27330	121328..123325	-	1998	WP_073519973.1	ParB/RepB/Spo0J family partition protein	-
RG61_RS27335	123395..123637	-	243	WP_073519974.1	DUF905 domain-containing protein	-
RG61_RS27340	123689..124246	-	558	WP_073519975.1	single-stranded DNA-binding protein	-
RG61_RS29255	124278..124367	-	90	WP_072223070.1	single-stranded DNA-binding protein	-
RG61_RS29260	124580..124801	+	222	WP_123006580.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeI / faeH / faeF / faeE / faeD / faeC / pic	1..146496	146496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5992.17 Da
• Isoelectric Point: 8.7840

>T51417 WP_073519970.1 NZ_CP010214:c119606-119445 [Escherichia coli]
MKLPRNALIWCVLIVCITLLIFTGLTRNRLCEVRLKDGEREVAASLAYESNGK

Nucleotide

• Length: 162 bp

>T51417 NZ_CP010214:c119606-119445 [Escherichia coli]
ATGAAACTGCCGCGAAACGCCCTTATCTGGTGCGTATTGATCGTGTGTATCACACTCTTGATATTCACAGGTCTGACCCG
GAATCGCTTATGTGAAGTCCGGCTGAAGGACGGAGAAAGGGAGGTCGCGGCAAGCCTGGCTTACGAATCCAACGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 178 bp

>AT51417 NZ_CP010214:c119836-119659 [Escherichia coli]
GATTATTTTCGGGGAAAGTGATGGCAGTGGTCAGCGTCGGTCCGTATAGGGTATGATGTGCCCGGCGCTTGAGGCTTTCT
GCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTATTCATTAACCCA
CGAGGCCCCCTGATGTCT

Structures

Antitoxin