Detailed information of TA system TA051411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4825520..4826122
• Replicon: chromosome
• Accession: NZ_CP010213
• Organism: Escherichia coli strain M15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG61_RS25100
• Protein ID: WP_000897305.1
• Coordinates: 4825811..4826122 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG61_RS25095
• Protein ID: WP_000356397.1
• Coordinates: 4825520..4825810 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG61_RS25060	4821067..4821702	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG61_RS25065	4821699..4822628	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG61_RS28455	4822775..4823035	-	261	WP_001508141.1	hypothetical protein	-
RG61_RS25070	4823027..4824007	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
RG61_RS25080	4824236..4824478	-	243	WP_001086388.1	hypothetical protein	-
RG61_RS25085	4824697..4824915	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG61_RS25095	4825520..4825810	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG61_RS25100	4825811..4826122	-	312	WP_000897305.1	hypothetical protein	Toxin
RG61_RS25105	4826351..4827259	+	909	WP_001162704.1	alpha/beta hydrolase	-
RG61_RS25110	4827323..4828312	-	990	WP_000080784.1	fatty acid biosynthesis protein FabY	-
RG61_RS25115	4828309..4828746	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG61_RS25120	4828743..4829615	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG61_RS25125	4829609..4830208	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4823027..4824007	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51411 WP_000897305.1 NZ_CP010213:c4826122-4825811 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51411 NZ_CP010213:c4826122-4825811 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51411 WP_000356397.1 NZ_CP010213:c4825810-4825520 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51411 NZ_CP010213:c4825810-4825520 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7