Detailed information of TA system TA051384

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28452..28878
• Replicon: plasmid C
• Accession: NZ_CP010209
• Organism: Escherichia coli strain M11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG60_RS27590
• Protein ID: WP_001312861.1
• Coordinates: 28452..28610 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	28654..28878 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG60_RS27540	23503..23730	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
RG60_RS27545	23824..24510	-	687	WP_073520017.1	PAS domain-containing protein	-
RG60_RS27550	24701..25084	-	384	WP_000124981.1	relaxosome protein TraM	-
RG60_RS27560	25361..26008	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
RG60_RS27565	26305..27126	-	822	WP_073520018.1	DUF945 domain-containing protein	-
RG60_RS27570	27244..27531	-	288	WP_000107535.1	hypothetical protein	-
RG60_RS27575	27556..27762	-	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG60_RS28505	27764..27952	-	189	WP_032141200.1	hypothetical protein	-
RG60_RS27590	28452..28610	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	28654..28878	-	225	NuclAT_0	-	Antitoxin
-	28654..28878	-	225	NuclAT_0	-	Antitoxin
-	28654..28878	-	225	NuclAT_0	-	Antitoxin
-	28654..28878	-	225	NuclAT_0	-	Antitoxin
RG60_RS27600	28890..29609	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
RG60_RS27605	29606..30040	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
RG60_RS27610	30095..32053	-	1959	WP_073520020.1	ParB/RepB/Spo0J family partition protein	-
RG60_RS27615	32118..32351	-	234	WP_001519997.1	DUF905 family protein	-
RG60_RS27620	32407..32946	-	540	WP_009426937.1	single-stranded DNA-binding protein	-
RG60_RS27625	32972..33178	-	207	WP_073520019.1	single-stranded DNA-binding protein	-
RG60_RS27630	33180..33368	-	189	WP_032141200.1	hypothetical protein	-
RG60_RS29220	33291..33515	+	225	WP_013279293.1	hypothetical protein	-
RG60_RS27635	33419..33646	-	228	WP_071594011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61005	61005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51384 WP_001312861.1 NZ_CP010209:c28610-28452 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51384 NZ_CP010209:c28610-28452 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT51384 NZ_CP010209:c28878-28654 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin