Detailed information of TA system TA051381

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108802..109193
• Replicon: plasmid A
• Accession: NZ_CP010207
• Organism: Escherichia coli strain M11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG60_RS26905
• Protein ID: WP_073519970.1
• Coordinates: 109032..109193 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	108802..108979 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG60_RS29195	103837..104058	-	222	WP_123006580.1	hypothetical protein	-
RG60_RS29200	104271..104360	+	90	WP_072223070.1	single-stranded DNA-binding protein	-
RG60_RS26870	104392..104949	+	558	WP_073519975.1	single-stranded DNA-binding protein	-
RG60_RS26875	105001..105243	+	243	WP_073519974.1	DUF905 domain-containing protein	-
RG60_RS26880	105313..107310	+	1998	WP_073519973.1	ParB/RepB/Spo0J family partition protein	-
RG60_RS26885	107351..107785	+	435	WP_097455818.1	conjugation system SOS inhibitor PsiB	-
RG60_RS26890	107782..108511	+	730	Protein_131	plasmid SOS inhibition protein A	-
RG60_RS26895	108508..108822	+	315	WP_073519971.1	theronine dehydrogenase	-
-	108802..108979	+	178	NuclAT_0	-	Antitoxin
-	108802..108979	+	178	NuclAT_0	-	Antitoxin
-	108802..108979	+	178	NuclAT_0	-	Antitoxin
-	108802..108979	+	178	NuclAT_0	-	Antitoxin
RG60_RS26900	108803..108954	-	152	Protein_133	protein sok	-
RG60_RS26905	109032..109193	+	162	WP_073519970.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG60_RS28490	109301..109741	+	441	WP_149025694.1	hypothetical protein	-
RG60_RS29205	109926..110337	-	412	Protein_136	hypothetical protein	-
RG60_RS29210	110374..110583	-	210	Protein_137	hypothetical protein	-
RG60_RS26920	110515..110644	+	130	Protein_138	single-stranded DNA-binding protein	-
RG60_RS26925	110634..110978	+	345	WP_073519969.1	hypothetical protein	-
RG60_RS26930	111122..111943	+	822	WP_073519968.1	DUF945 domain-containing protein	-
RG60_RS26935	112239..112748	-	510	WP_073519994.1	transglycosylase SLT domain-containing protein	-
RG60_RS26940	113172..113558	+	387	WP_073519967.1	relaxosome protein TraM	-
RG60_RS26945	113747..114169	+	423	WP_073519966.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pic / faeC / faeD / faeE / faeF / faeH / faeI	1..138950	138950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5992.17 Da
• Isoelectric Point: 8.7840

>T51381 WP_073519970.1 NZ_CP010207:109032-109193 [Escherichia coli]
MKLPRNALIWCVLIVCITLLIFTGLTRNRLCEVRLKDGEREVAASLAYESNGK

Nucleotide

• Length: 162 bp

>T51381 NZ_CP010207:109032-109193 [Escherichia coli]
ATGAAACTGCCGCGAAACGCCCTTATCTGGTGCGTATTGATCGTGTGTATCACACTCTTGATATTCACAGGTCTGACCCG
GAATCGCTTATGTGAAGTCCGGCTGAAGGACGGAGAAAGGGAGGTCGCGGCAAGCCTGGCTTACGAATCCAACGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 178 bp

>AT51381 NZ_CP010207:108802-108979 [Escherichia coli]
GATTATTTTCGGGGAAAGTGATGGCAGTGGTCAGCGTCGGTCCGTATAGGGTATGATGTGCCCGGCGCTTGAGGCTTTCT
GCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTATTCATTAACCCA
CGAGGCCCCCTGATGTCT

Structures

Antitoxin