Detailed information of TA system TA051376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3972102..3972704
• Replicon: chromosome
• Accession: NZ_CP010206
• Organism: Escherichia coli strain M11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG60_RS20675
• Protein ID: WP_000897305.1
• Coordinates: 3972393..3972704 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG60_RS20670
• Protein ID: WP_000356397.1
• Coordinates: 3972102..3972392 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG60_RS20635	3967649..3968284	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG60_RS20640	3968281..3969210	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG60_RS28320	3969357..3969617	-	261	WP_001508141.1	hypothetical protein	-
RG60_RS20645	3969609..3970589	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
RG60_RS20655	3970818..3971060	-	243	WP_001086388.1	hypothetical protein	-
RG60_RS20660	3971279..3971497	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG60_RS20670	3972102..3972392	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG60_RS20675	3972393..3972704	-	312	WP_000897305.1	hypothetical protein	Toxin
RG60_RS20680	3972933..3973841	+	909	WP_001162704.1	alpha/beta hydrolase	-
RG60_RS20685	3973905..3974894	-	990	WP_000080784.1	fatty acid biosynthesis protein FabY	-
RG60_RS20690	3974891..3975328	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG60_RS20695	3975325..3976197	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RG60_RS20700	3976191..3976790	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3969609..3970589	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51376 WP_000897305.1 NZ_CP010206:c3972704-3972393 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51376 NZ_CP010206:c3972704-3972393 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51376 WP_000356397.1 NZ_CP010206:c3972392-3972102 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51376 NZ_CP010206:c3972392-3972102 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7