Detailed information of TA system TA051350

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4670789..4671160
• Replicon: chromosome
• Accession: NZ_CP010200
• Organism: Escherichia coli strain M10

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: RG59_RS24190
• Protein ID: WP_001317028.1
• Coordinates: 4670966..4671160 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4670789..4670967 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG59_RS24165	4666744..4668117	+	1374	WP_000123720.1	ATP-dependent RNA helicase DbpA	-
RG59_RS24170	4668246..4669181	-	936	WP_001676520.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
RG59_RS24175	4669233..4670468	-	1236	WP_000040852.1	site-specific integrase	-
RG59_RS24180	4670470..4670685	-	216	WP_000079604.1	excisionase XisR	-
RG59_RS24185	4670764..4670973	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	4670789..4670967	+	179	NuclAT_0	-	Antitoxin
-	4670789..4670967	+	179	NuclAT_0	-	Antitoxin
-	4670789..4670967	+	179	NuclAT_0	-	Antitoxin
-	4670789..4670967	+	179	NuclAT_0	-	Antitoxin
RG59_RS24190	4670966..4671160	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
RG59_RS24195	4671217..4672026	-	810	WP_000166319.1	recombination protein RecT	-
RG59_RS24200	4672019..4674619	-	2601	WP_073553840.1	exodeoxyribonuclease VIII	-
RG59_RS24205	4674721..4674996	-	276	WP_000632297.1	protein RacC	-
RG59_RS24210	4675071..4675241	-	171	WP_001352098.1	conserved protein, Rac prophage	-
RG59_RS24215	4675241..4675462	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T51350 WP_001317028.1 NZ_CP010200:c4671160-4670966 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T51350 NZ_CP010200:c4671160-4670966 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT51350 NZ_CP010200:4670789-4670967 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin