Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3500329..3500870 Replicon chromosome
Accession NZ_CP010200
Organism Escherichia coli strain M10

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag RG59_RS18100 Protein ID WP_000615976.1
Coordinates 3500329..3500607 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RG59_RS18105 Protein ID WP_000729704.1
Coordinates 3500610..3500870 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG59_RS18080 3497911..3498489 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
RG59_RS18090 3498695..3499462 + 768 WP_000333380.1 class II glutamine amidotransferase -
RG59_RS18095 3499433..3500173 - 741 WP_001225679.1 murein L,D-transpeptidase -
RG59_RS18100 3500329..3500607 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RG59_RS18105 3500610..3500870 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RG59_RS18110 3501056..3501829 + 774 WP_000543899.1 C40 family peptidase -
RG59_RS18115 3501886..3502242 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RG59_RS18120 3502235..3502513 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RG59_RS18125 3502618..3504357 - 1740 WP_001306917.1 flagellar biosynthesis protein FlhA -
RG59_RS18130 3504317..3505087 + 771 WP_001365758.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T51344 WP_000615976.1 NZ_CP010200:c3500607-3500329 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T51344 NZ_CP010200:c3500607-3500329 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT51344 WP_000729704.1 NZ_CP010200:c3500870-3500610 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT51344 NZ_CP010200:c3500870-3500610 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References