Detailed information of TA system TA051339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2641426..2642028
• Replicon: chromosome
• Accession: NZ_CP010200
• Organism: Escherichia coli strain M10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RG59_RS13755
• Protein ID: WP_000897305.1
• Coordinates: 2641426..2641737 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RG59_RS13760
• Protein ID: WP_000356397.1
• Coordinates: 2641738..2642028 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG59_RS13730	2637340..2637939	+	600	WP_001295269.1	glucose-1-phosphatase	-
RG59_RS13735	2637933..2638805	+	873	WP_000920762.1	virulence factor BrkB family protein	-
RG59_RS13740	2638802..2639239	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
RG59_RS13745	2639236..2640225	+	990	WP_000080784.1	fatty acid biosynthesis protein FabY	-
RG59_RS13750	2640289..2641197	-	909	WP_001299484.1	alpha/beta hydrolase	-
RG59_RS13755	2641426..2641737	+	312	WP_000897305.1	hypothetical protein	Toxin
RG59_RS13760	2641738..2642028	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
RG59_RS13770	2642633..2642851	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
RG59_RS13775	2643070..2643312	+	243	WP_001087409.1	hypothetical protein	-
RG59_RS13785	2643642..2644571	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RG59_RS13790	2644568..2645203	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
RG59_RS13795	2645200..2646102	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T51339 WP_000897305.1 NZ_CP010200:2641426-2641737 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T51339 NZ_CP010200:2641426-2641737 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT51339 WP_000356397.1 NZ_CP010200:2641738-2642028 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT51339 NZ_CP010200:2641738-2642028 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7