Detailed information of TA system TA051318

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14827..15218
• Replicon: plasmid A
• Accession: NZ_CP010197
• Organism: Escherichia coli strain M9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: RG58_RS26960
• Protein ID: WP_073519970.1
• Coordinates: 15057..15218 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	14827..15004 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG58_RS28950	9911..10132	-	222	WP_154813206.1	hypothetical protein	-
RG58_RS29300	10197..10346	-	150	WP_157920373.1	hypothetical protein	-
RG58_RS26925	10417..10974	+	558	WP_073519975.1	single-stranded DNA-binding protein	-
RG58_RS26930	11026..11268	+	243	WP_073519974.1	DUF905 domain-containing protein	-
RG58_RS26935	11338..13335	+	1998	WP_073519973.1	ParB/RepB/Spo0J family partition protein	-
RG58_RS26940	13376..13810	+	435	WP_097455818.1	conjugation system SOS inhibitor PsiB	-
RG58_RS26945	13807..14536	+	730	Protein_20	plasmid SOS inhibition protein A	-
RG58_RS26950	14533..14847	+	315	WP_073519971.1	theronine dehydrogenase	-
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
-	14827..15004	+	178	NuclAT_0	-	Antitoxin
RG58_RS26960	15057..15218	+	162	WP_073519970.1	type I toxin-antitoxin system Hok family toxin	Toxin
RG58_RS28330	15392..15766	+	375	WP_171769349.1	hypothetical protein	-
RG58_RS28960	15951..16362	-	412	Protein_24	hypothetical protein	-
RG58_RS26980	16659..17003	+	345	WP_073519969.1	hypothetical protein	-
RG58_RS26985	17147..17968	+	822	WP_073519968.1	DUF945 domain-containing protein	-
RG58_RS26990	18264..18773	-	510	WP_073519994.1	transglycosylase SLT domain-containing protein	-
RG58_RS26995	19197..19583	+	387	WP_073519967.1	relaxosome protein TraM	-
RG58_RS27000	19772..20194	+	423	WP_073519966.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / faeC / faeD / faeE / faeF / faeH / faeI	1..146524	146524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5992.17 Da
• Isoelectric Point: 8.7840

>T51318 WP_073519970.1 NZ_CP010197:15057-15218 [Escherichia coli]
MKLPRNALIWCVLIVCITLLIFTGLTRNRLCEVRLKDGEREVAASLAYESNGK

Nucleotide

• Length: 162 bp

>T51318 NZ_CP010197:15057-15218 [Escherichia coli]
ATGAAACTGCCGCGAAACGCCCTTATCTGGTGCGTATTGATCGTGTGTATCACACTCTTGATATTCACAGGTCTGACCCG
GAATCGCTTATGTGAAGTCCGGCTGAAGGACGGAGAAAGGGAGGTCGCGGCAAGCCTGGCTTACGAATCCAACGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 178 bp

>AT51318 NZ_CP010197:14827-15004 [Escherichia coli]
GATTATTTTCGGGGAAAGTGATGGCAGTGGTCAGCGTCGGTCCGTATAGGGTATGATGTGCCCGGCGCTTGAGGCTTTCT
GCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTATTCATTAACCCA
CGAGGCCCCCTGATGTCT

Structures

Antitoxin