Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 26990..27541 Replicon plasmid B
Accession NZ_CP010193
Organism Escherichia coli strain M8

Toxin (Protein)


Gene name yafQ Uniprot ID A0A7H0EV41
Locus tag RG57_RS26140 Protein ID WP_073535404.1
Coordinates 26990..27274 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A7H0EV42
Locus tag RG57_RS26145 Protein ID WP_073535405.1
Coordinates 27281..27541 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG57_RS26100 22697..23041 + 345 WP_073535397.1 hypothetical protein -
RG57_RS26105 23108..23533 + 426 WP_073535398.1 H-NS histone family protein -
RG57_RS26110 23545..23868 + 324 WP_073535429.1 hypothetical protein -
RG57_RS26115 24138..24923 + 786 WP_073535399.1 SprT-like domain-containing protein -
RG57_RS26120 24925..25497 + 573 WP_073535400.1 hypothetical protein -
RG57_RS26125 25494..26129 + 636 WP_073535401.1 hypothetical protein -
RG57_RS26130 26156..26509 + 354 WP_073535402.1 hypothetical protein -
RG57_RS27645 26549..26701 + 153 WP_187661356.1 hypothetical protein -
RG57_RS26135 26712..26915 + 204 WP_073535403.1 hypothetical protein -
RG57_RS26140 26990..27274 - 285 WP_073535404.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
RG57_RS26145 27281..27541 - 261 WP_073535405.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
RG57_RS26150 27714..27929 + 216 WP_073535406.1 hypothetical protein -
RG57_RS26155 27929..28429 + 501 WP_073535407.1 thermonuclease family protein -
RG57_RS26160 28435..28743 + 309 WP_073535408.1 hypothetical protein -
RG57_RS26165 28756..28992 + 237 WP_073535409.1 hypothetical protein -
RG57_RS26170 29094..29381 + 288 WP_073535410.1 hypothetical protein -
RG57_RS26175 29425..29730 + 306 WP_073535411.1 hypothetical protein -
RG57_RS26180 29741..30145 + 405 WP_073535412.1 hypothetical protein -
RG57_RS26185 30189..30635 + 447 WP_073535413.1 hypothetical protein -
RG57_RS26190 30635..31036 + 402 WP_073535414.1 hypothetical protein -
RG57_RS26195 31037..31240 + 204 WP_073535415.1 hypothetical protein -
RG57_RS26200 31252..31557 + 306 WP_073535416.1 hypothetical protein -
RG57_RS26205 31547..31780 + 234 WP_073535417.1 hypothetical protein -
RG57_RS27650 32206..32367 + 162 WP_172687973.1 hypothetical protein -
RG57_RS27655 32371..32508 + 138 WP_154674501.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40584 40584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-93)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11136.92 Da        Isoelectric Point: 10.1201

>T51296 WP_073535404.1 NZ_CP010193:c27274-26990 [Escherichia coli]
MTRKREIEYSGQFQKDVKKAQKRNKDMAKLKTLITLLIEDKLPLPAIYKDHPLQGDYKGYRDAHIEPDWLLIYKISDTKL
RFERTGTHSDLFKG

Download         Length: 285 bp

>T51296 NZ_CP010193:c27274-26990 [Escherichia coli]
ATGACCAGAAAAAGGGAAATTGAATACTCAGGACAGTTTCAGAAAGATGTGAAGAAAGCTCAGAAGCGTAATAAGGATAT
GGCAAAGCTAAAAACCCTTATTACGCTCTTAATTGAAGATAAGCTACCGTTACCAGCAATATATAAAGATCACCCGTTAC
AAGGTGATTATAAAGGTTACAGGGATGCACATATCGAGCCTGATTGGTTATTGATTTACAAAATTTCAGATACCAAACTT
CGTTTTGAACGTACAGGCACACATTCAGATCTGTTTAAAGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9195.58 Da        Isoelectric Point: 5.0628

>AT51296 WP_073535405.1 NZ_CP010193:c27541-27281 [Escherichia coli]
MAANAFVRARIDEALKNEAADVLAGMGLTVSDLVRITLTKVAVEKALPFDMRIPNRTTAATIAASEKGVDVHRADDANDL
FEKLGI

Download         Length: 261 bp

>AT51296 NZ_CP010193:c27541-27281 [Escherichia coli]
ATGGCCGCAAACGCTTTTGTTCGTGCTCGCATCGATGAAGCACTGAAAAACGAAGCCGCTGATGTGCTGGCGGGTATGGG
GTTAACCGTGTCAGACCTTGTAAGGATAACCCTTACAAAAGTAGCTGTTGAAAAGGCGCTGCCGTTTGATATGCGCATAC
CTAACAGAACGACGGCGGCGACCATCGCAGCCAGTGAAAAAGGCGTTGATGTACACCGCGCAGACGATGCTAACGACCTG
TTTGAAAAATTGGGTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0EV41

Antitoxin

Source ID Structure
AlphaFold DB A0A7H0EV42

References