Detailed information of TA system TA051292

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148374..148800
• Replicon: plasmid A
• Accession: NZ_CP010192
• Organism: Escherichia coli strain M8

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RG57_RS25835
• Protein ID: WP_001312861.1
• Coordinates: 148374..148532 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	148576..148800 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG57_RS25790	143685..143912	-	228	WP_032246093.1	conjugal transfer relaxosome protein TraY	-
RG57_RS25795	144006..144692	-	687	WP_073535353.1	PAS domain-containing protein	-
RG57_RS25800	144883..145266	-	384	WP_073535354.1	relaxosome protein TraM	-
RG57_RS25810	145552..146199	+	648	WP_149025818.1	transglycosylase SLT domain-containing protein	-
RG57_RS25815	146496..147317	-	822	WP_001234469.1	DUF945 domain-containing protein	-
RG57_RS25820	147440..147727	-	288	WP_000107535.1	hypothetical protein	-
RG57_RS27005	147729..147926	+	198	Protein_143	hypothetical protein	-
RG57_RS25835	148374..148532	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	148576..148800	-	225	NuclAT_0	-	Antitoxin
-	148576..148800	-	225	NuclAT_0	-	Antitoxin
-	148576..148800	-	225	NuclAT_0	-	Antitoxin
-	148576..148800	-	225	NuclAT_0	-	Antitoxin
RG57_RS27635	148612..148800	+	189	WP_001496304.1	hypothetical protein	-
RG57_RS25845	148812..149531	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
RG57_RS25850	149528..149962	-	435	Protein_147	conjugation system SOS inhibitor PsiB	-
RG57_RS25855	150017..151975	-	1959	WP_073535355.1	ParB/RepB/Spo0J family partition protein	-
RG57_RS25860	152034..152267	-	234	WP_073535356.1	DUF905 domain-containing protein	-
RG57_RS25865	152323..152850	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
RG57_RS25880	153323..153589	-	267	WP_072254135.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..162720	162720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T51292 WP_001312861.1 NZ_CP010192:c148532-148374 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T51292 NZ_CP010192:c148532-148374 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT51292 NZ_CP010192:c148800-148576 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AAATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin